采用响应面试验对1株γ-聚谷氨酸产生菌的发酵培养基进行优化,得到的产γ-PGA发酵培养基组合为:NaCl15g/L、豆粕53g/L、柠檬酸钠16g/L、谷氨酸钠40g/L、NH4Cl4.6g/L、K2HPO40.625g/L、MgSO41.25g/L、MnSO40.35g/L、CaCl20.2g/L,在此条件下,γ-PGA发酵产率为25.81g/L,比优化前提高了约1.5倍。

Response surface experiment was designed to identify optimum medium. The optimum medium for Bacillus amyloliquefaciens L536 was composed of:NaCl 15 g/L,soybean meal 53 g/L,sodium citrate 16 g/L,sodium hydrogen glutamate 40 g/L,NH4Cl 0.46 g/L,K2HPO4 0.625 g/L,MgSO4·7H2O 1.25 g/L,MnSO4·H2O 0.35 g/L,CaCl2·2H2O 0.2 g/L. Under these conditions,the yield of γ-PGA was 25.81 g/L,increased for about 1.5 times more than before. In order to obtain the optimum fermentation medium for γ-PGA production,the method of response surface experimental design,experimental procedure and results are discussed in this paper.