以酸性蛋白酶水解带鱼脊骨,在酶用量为1/150(g/gprotein),pH2.67,温度45℃,料水比1:4.38(g/mL),酶解7h条件下制得具有降压活性的酶解液,再以截留分子质量为3ku的超滤膜超滤制备得到分子质量小于3ku的降血压肽(ACEIP),其降血压活性ACEIC50达到0.727mg/mL。以得到的降血压肽饲喂原发性高血压大鼠(SHR),结果表明,带鱼脊骨ACEIP有降低原发性高血压大鼠血压的作用,并且呈现了一定的量效关系。长期饲喂低剂量组(10mg/kg体重)使SHR血压下降15mmHg,高剂量组(50mg/kg体重)组对SHR降压幅度达到20mmHg,并且降压效果稳定。

Ribbonfish (Trichiurus haumela) backbone was hydrolyzed by acid protease under the optimal conditions of pH 2.67,45℃,substrate to water ratio of 1:4.38 g/mL,enzyme to substrate of 1/150 g/g protein for 7 h,and then the hydrolysate was separated by ultra filtration of 3 ku to get angiotensin I converting enzyme inhibitory peptide (ACEIP).The ACEIP had ACEIC50 of 0.727 mg/mL.Animal experiments were carried out including single administration and long term administration of spontaneously hypertensive rat (SHR) to determine the anti-hypertensive activity.The results showed that ACEIP from ribbonfish backbone had the anti-hypertensive function,and showed certain dose-effect relationship.The low and high dose treatments reduced the blood pressure of 15 and 20 mmHg of SHR in the long term administration experiment,respectively.