研究了水提醇沉大麦麦芽根多糖的抗氧化活性。采用水提旋蒸浓缩醇沉法提取并精制多糖,用体积分数50%和80%乙醇分段醇析分离得2种粗多糖BCP50和BCP80;用分光光度法研究了2种粗多糖在不同体系中对羟自由基、DPPH自由基的清除作用、还原力以及用油脂氧化仪测定其抗氧化活性。结果表明:BCP50和BCP80多糖具有清除羟基自由基、DPPH自由基和还原力以及抗油脂氧化能力,多糖BCP80的作用效果强于BCP50。

Antioxidant activities of polysaccharides from barley malt roots by aqueous-extraction and alcohol precipitation were studied.The crude extracted polysaccharides were classified by 50% and 80% of ethanol into BCP50 and BCP80.The antioxidant capacity of the polysaccharides was measured by spectrophotometry on its ability of scavenging 1.1-diphenyl-2-picrylhydrazyl(DPPH·),hydroxyl radical,reducing power and the antioxidant capability on fat and oils.The results revealed that BCP80 and BCP50 had free radical-scavenging ability and BCP80 was better than BCP50.