采用超声波辅助法对葫芦巴香精油提取进行单因素和正交试验研究,并对香精油做抑菌性能研究。结果表明,超声波提取葫芦巴香精油优化的提取工艺条件为:浸泡时间20 min、料液比1∶4(g∶mL)、超声时间20min、超声功率50 W,在此条件下香精油含量达2.61%;影响香精油提取率因素的主次顺序为:超声功率>料液比>浸泡时间>超声时间;葫芦巴香精油对枯草芽胞杆菌、大肠杆菌、金黄葡萄球菌、青霉、毛霉种菌有较强的抑制效果,对黑曲霉也有一定的抑制作用,对其最低抑制浓度分别为:枯草芽胞杆菌0.4 g/L,大肠杆菌、金黄葡萄球菌和青霉为0.5 g/L,毛霉为0.6 g/L,黑曲霉为1.0 g/L。

The extracting conditions and anti-microbial activity of essential oil in Fenugreek were studied.The results showed that the optimum extraction conditions were soaking time for 20min,ratio to solvent 1∶4(g∶mL),extracting time for 20min and ultrasonic power 50 W.Under this extracting conditions,the yield of essential oil is to 2.61%.Essential oil in Fenugreek had strong anti-microbial activity,and the Minimum Inhibitory Concentration(MIC)of the essential oil was 0.4g/L against Bacillus subtilis,1.0 g/L against Aspegillus niger,0.6 g/L against Actinomucor sp.,and 0.5 g/L against E.coli.,S.aureus,Penicillium sp.