建立了一种利用反相高效液相色谱法测定发酵液中β-聚苹果酸(PMLA)含量的方法。以A(0.01mol/LKH2PO4)∶B(乙腈)=95∶5(体积化)为流动相,采用色谱柱Synergi4uPolar-RP(4.6×250mm,5μm);Agilent1200seires液相色谱系统测定发酵液酸水解前后的苹果酸含量,两者差值间接反映发酵液中聚苹果酸的含量。结果显示,目的峰和杂峰在8min内可以得到完全分离,定量的相对标准偏差为0.6%～1.5%,回收率为98.2%～100%。该法准确度高,重现性好,可作为发酵液中聚苹果酸的检测方法。

Optimization of conditions for quantitative determination of the content of poly(β-malic acid)in fermentation broth was established by RP-HPLC.Methods:A(0.01 mol/L KH2PO4)∶B(methyl cyanide)= 95∶5 as mobile phase,using column Synergi 4u Polar-RP(4.6 × 250 mm,5 μm),Agilent 1200 series liquid color spectrum system to measure the contents of malic acid before and after acid hydrolysis,and their difference in value reports the content of poly(β-malic acid)in fermentation broth indirectly.Results showed that the target peaks and impure peaks were completely separated within 8 minutes,quantitative relative standard deviation is 0.6%～1.5% and the recovery is 98.2%～100 %.This method can be used to detect poly(β-malic acid)in fermentation broth with high accuracy and good reproducibility.