以不同的固定化材料对耐盐酵母进行固定化,从中选择适宜的固定化载体。采用正交实验确定了适宜固定化的条件,并采用SPSS统计软件进行极差和方差分析。结果表明,海藻酸钠-氯化钙固定化耐盐酵母优于琼脂、明胶固定化耐盐酵母,当海藻酸钠浓度4g/100mL,CaCl2浓度为1.4mol/L,发酵温度为28℃时,耐盐酵母的固定化效果较好。

In order to get the best carrier,we used different materials to immobilize the salt-tolerant yeast.The optimal immobilization parameters were studied by orthogonal experiments,and range analysis and variance analysis were done by using SPSS.The final result showed the sodium alginate and calcium chloride were better than agar and gelatin in immobilization of the salt-resistance yeast.The optimum conditions were the concentration of sodium alginate 4 g/100 mL,calcium chloride 1.4 mol/L,at 28℃.Under this condition,the effect of immobilization was better.