建立了利用反相高效液相色谱法同时快速测定黄酒和葡萄酒中酒石酸、甲酸、苹果酸、乳酸、乙酸、柠檬酸、琥珀酸等7种有机酸的方法。采用的色谱柱为XB-C18(5μm,150mm×4.6mm),检测器为紫外检测器,波长210nm;确定的最优色谱条件是:0.02mol/LKH2PO4-乙腈(98∶2),pH2.3,流速1.0mL/min,柱温30℃,进样量10.0μL,冲洗方式为等度洗脱。利用此方法分析测定有机酸的工作曲线线性关系良好,回收率在83.17%～117.3%,变异系数在3.1%～5.8%。利用研究所构建的反相高效液相色谱法能够快速地对黄酒和葡萄酒中的有机酸进行测定,满足企业对产品质量进行实时监控。

This paper established a method using RP-HPLC simultaneously and rapidly detecting of tartaric acid,formic acid,malic acid,lactic acid,acetic acid,citric acid,succinic acid in rice wine and grape wine.The optimal separation conditions:the XB-C18(5μm,150mm × 4.6mm)ultraviolet detector,wavelength 210 nm,mobile phase 0.02M KH2PO4-acetonitrile(98:2),pH 2.3,flow rate 1.0 ml/min,column temperature 30℃,injection of 10.0μl,Isocratic elution.The recovery rate ranged from 83.17 to 117.3%,and the relative standard deviations are 3.1%～5.8%.Reverse-phase high performance liquid chromatography can determine the organic acid in yellow rice wine and wine rapidly,which satisfy enterprises needs for real-time monitoring and quality control.