应用琼脂平板扩散法、DPPH.法、结晶紫法、邻苯三酚自氧化法,测定五味子提取物对大肠杆菌、金黄色葡萄球菌、乙型副伤寒沙门氏菌的抑菌活性及清除1,1-二苯基-2-苦苯肼自由基(DPPH.)、羟自由基(.OH)、超氧阴离子(O2-.)的能力。结果表明,五味子提取物对大肠杆菌、金黄色葡球菌、乙型副伤寒沙门氏菌3种食品常见致病菌均具有强的抑菌作用,抑菌圈直径均在20mm以上。其中,五味子乙醇提取物对大肠杆菌、金黄色葡球菌、沙门氏菌的抑菌圈和MIC及对DPPH.、.OH、O2-.清除活性EC50分别为34.8mm,28.6mm,23.2mm,0.1250g/mL,0.0313g/mL,0.1250g/mL,0.69mg/mL,0.78mg/mL,0.57mg/mL;蒸馏水提取物的抑菌圈和MIC清除率分别为25.4mm,23.0mm,23.4mm,0.1250g/mL,0.0625g/mL,0.1250g/mL,0.72mg/mL,0.63mg/mL,0.61mg/mL。说明五味子提取物具有明显的抗菌和抗氧化作用。

To study the effects of Antibacterial and anti-oxidation of Extracts from Kadsura longipedunculata.The inhibition effect of the extracts from K.longipedunculata against E.coli,S.aureus,S.paratyphi and its scavenging abilities to 1,1-2-phenyl-2-bitter phenylhydrazine radical(DPPH·),hydroxyl radical(·OH),superoxide anion(O2-·)were respectively determined via agar plate diffusion method,DPPH· method and pyrogallol auto-oxidation method.The extracts from K.longipedunculata,with its inhibition zone diameters larger than 20mm,have strong inhibition effect to three kinds of food pathogenic bacteria:E.coli,S.aureus and S.paratyphi.Inhibition zone diameters of inhibition and MIC of ethanol extract against E.coli,S.aureus,S.paratyphi were 34.8 mm,28.6mm,23.2mm,0.1250g/mL,0.0313 g /mL,0.1250 g /mL respectively,and scavenging abilities to 1,1-2-phenyl-2-bitter phenylhydrazine radical(DPPH·),hydroxyl radical(·OH),superoxide anion(O2-·)were 0.69 mg/mL,0.78 mg/mL,0.57 mg/mL.Distilled water extract of K.longipedunculata and MIC against E.coli,S.aureus,S.paratyphi were 25.4 mm,23.0 mm,23.4 mm,0.125 0 g/mL,0.0625g /mL,0.1250 g /mL respectively and scavenging abilities of 1,1-2-phenyl-2-bitter phenylhydrazine radical(DPPH ·),hydroxyl radical(·OH),superoxide anion(O2-·)were 0.72 mg/mL,0.63 mg/mL,0.61 mg/mL.K.longipedunculata extract has strong anti-oxidation and inhibition effect to bacteria.