研究了采用大孔吸附树脂富集茶多酚中低苦涩味儿茶素的工艺。通过静态吸附和解吸实验,对9种树脂进行了筛选,优选得到ADS-17树脂,考察了茶多酚浓度对该树脂吸附量、吸附率的影响,以及其等温吸附曲线、动态吸附和乙醇浓度梯度洗脱特性。结果表明:ADS-17树脂对低苦涩味表没食子儿茶素(EGC)的饱和吸附量为15.44 mg/g,所得制品的EGC含量达43.84%,为富集前茶多酚中EGC含量的7倍,并且富集所得制品中EGC占儿茶素总量的64.57%,取代表没食子儿茶素没食子酸酯(EGCG)成为主要的呈味成分。

Macroporous adsorption resin was used for the enrichment of low bitter and low astringent catechins from green tea polyphenol.ADS-17 was selected from nine macroporous adsorption resin by testing the ratio of EGC/ EGCG in the solution of static adsorption and elution,investigating the influences of concentration of green tea polyphenol solution(TP) and adsorption time on the adsorption capability of the tested resin.The dynamic sorption date and alcohol gradient elution date of the selected resin were also investigated.The saturated adsorption capacity of ADS-17 for EGC was 15.44 mg/g,the content of EGC in final product was 43.84 %,which was 7 times higher than the initial extract of 6.22 % of EGC.The proportion of EGC in total catechins was 64.57 %,which replaced EGCG and became the major flavor compound.The proposed method in this paper is simple and feasible for the enrichment of EGC,which is the major compound of low bitter and low astringent catenhins.The method has great potential for industrial application.