食品与发酵工业

Alcalase碱性蛋白酶水解厚壳贻贝蛋白

  • 戴志远 ,
  • 张婷 ,
  • 张燕平 ,
  • 卢延斌 ,
  • 张艳萍
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网络出版日期: 2010-08-25

Study on Hydrolysis of Mytilus coruscus Protein with Alcalase Protease

  • Dai Zhi-yuan ,
  • Zhang Ting ,
  • Zhang Yan-ping ,
  • Lu Yan-bin ,
  • Zhang Yan-ping
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Online published: 2010-08-25

摘要

以厚壳贻贝为原料,Alcalase2.4L碱性蛋白酶为酶源,在单因素试验的基础上,以还原力A700为指标,运用响应面分析法(RSM)得到Alcalase2.4L酶解厚壳贻贝蛋白的优化条件为:底物浓度[S]3.2%、[E]/[S]2.12%、温度55.54℃、pH8.66、时间2h,该条件下制备的酶解物的还原能力为0.587。同时证明了该酶解物具有显著清除DPPH自由基活性和较强的超氧阴离子自由基清除能力。

本文引用格式

戴志远 , 张婷 , 张燕平 , 卢延斌 , 张艳萍 . Alcalase碱性蛋白酶水解厚壳贻贝蛋白[J]. 食品与发酵工业, 2010 , 36(08) : 83 -88 . DOI: 10.13995/j.cnki.11-1802/ts.2010.08.032

Abstract

Alcalase2.4L were applied to hydrolyze the Mytilus coruscus protein.Based on the results of single-factor tests,reducing power A700 was chosen as the response values.Using Response Surface Methodology(RSM),the optimum enzyme hydrolysis conditions of Alcalase2.4L to achieve the maximum antioxidant ability were as follows:substrate concentration([S]) 3.20%,[E]/[S]2.12%,enzymolysis temperature 55.54℃,and enzymolysis time 2 hours.Under such conditions,reducibility of the hydrolysate reached 0.587,which also had strong scavenging activity of DPPH radical and superoxide anion radical.
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