为提高葡萄皮渣中白藜芦醇的含量,解决白藜芦醇产量低,生产成本高的问题,采用固态发酵法处理葡萄皮渣。运用正交实验法对固态发酵方案进行设计,再利用超声波法以及高效液相色谱技术(HPLC)分别对发酵后的葡萄皮渣中白藜芦醇进行提取和检测。试验结果表明,28℃下最优的固态发酵处理条件为:发酵液固比(m∶m)2.0∶1,发酵时间72h,碳源添加量1.5%,此条件下白藜芦醇得率可达1.846μg/g,是未经固态发酵葡萄皮渣中白藜芦醇含量的3.46倍。

For enhancing Resveratrol content of the grape skin residues,solving the low production of Resveratrol and the problem of high production costs,in this paper,the following contents were studied:1 applying single factor with the orthogonal solid-state fermentation to deal with the grape skin residues that produced wine by fermentation;2 using ultrasound and high-performance liquid chromatography to extract and detect the Resveratrol separately.The main results were as follows:at 28 ℃,the optimization of solid-state fermentation conditions were the ratio of solid to liquid of the fermentation was 1∶2.0(m∶m),the fermentation time was 72 h,carbon(glucose)amount was 0.15 g /g.The highest Resveratrol content may reach to 1.846 mg /L and was 3.46 times more than those not-fermenting.Solid-state fermentation treatment can significantly increase Resveratrol of grape skin residue in the yield.