样品中的虫草素、尿苷和腺苷用纯水经水浴80℃/30min提取,采用CALESILODS-100C18柱(5μm,4.6×250mm),在流动相为10mmol/LKH2PO3缓冲液(pH=6.0)∶甲醇=85∶15的色谱条件下分离,紫外260nm下检测。结果显示,虫草素、尿苷和腺苷的检测限分别为0.02μg/mL、0.01μg/mL和0.01μg/mL(以进样浓度计);方法的相对标准偏差分别为1.8%、0.68%和0.10%;方法的加标回收率分别为96.7%、96.4%和99.4%。实验所建立的方法简便、快速、结果准确、可靠,能应用于虫草制品中虫草素、尿苷和腺苷含量的测定。

The cordycepin,uridine and adenosine in samples were extracted by water at 80oC,separated with CALESIL ODS-100 Cl8 column(5μm,4.6×250 mm),10mmoL/L monopotassium phosphate(pH=6.0),methanol(85∶15,V/V)in mobile phase,and detected at UV 260nm.The detection limit of the three components was 0.02μg/mL,0.01μg/mL and 0.01μg/mL(count by concentration of sample injection).The recovery rates were 96.7% and 96.4% and 99.4%.Relative standard deviation were 1.8% and 0.68% and 0.10%.The method is simple,quick,accurate and can be applied for detection of cordycepin and Uridine and adenosine in foods.