采用缓冲液低温提取马氏珍珠贝中的糖蛋白,以硫酸铵对糖蛋白粗提液进行梯度分级沉淀,对各级分的组成和抗氧化活性进行了分析比较,并取硫酸铵浓度为80%～100%的沉淀组分经SephadexG-75层析纯化,得到糖蛋白PMGP,经SDS-PAGE及HPLC验证其纯度较高,分子质量约为11.6ku,蛋白含量95.08%,糖含量4.92%,具有较强的抗氧化活性。

The glycoprotein of Pinctada martensii was extracted with phosphate buffer in low temprature.The crude extract was then fractionated with 4 concentrations of ammonium sulfate solution.The composition and anti-oxidative property of the fractions were studied.Purified PMGP was prepared from the precipatate at 80%~100% saturation through Sephadex G-75 chromatography.SDS-PAGE and HPLC analysis showed that the PMGP was of high purity,and its molecular weight was about 11.6ku.The content of protein in PMGP was 95.08%,and sugar was 4.92%.It showed strong scavenging efficiency on DPPH radical and superoxide anion radical.