对酿酒酵母CWY132利用糖蜜为碳源,生物转化L-苯丙氨酸(L-Phe)生成2-苯乙醇的液-液两相分批补料培养工艺进行了研究。发现在以聚丙二醇(PPG1500)作为抽提剂的液-液两相培养中,采用分批补料方式添加糖蜜和L-Phe使2-苯乙醇产量明显提高。在0、4、8、12、24 h分别添加40 g/L糖蜜,4、8 h分别添加12 g/L、3g/L L-Phe的液-液两相分批补料培养中,2-苯乙醇产量最高达到9.03 g/L,其中抽提相中2-苯乙醇浓度22.5 g/L,比优化前的液-液两相单批培养中的产量4.82 g/L提高了87%。底物L-Phe的摩尔转化率达到0.82 mol/mol。

Two-phase fed-batch fermentation conditions for the production of 2-phenylethanol by Saccharomyces cerevisiae CWY132 in a molasses-based medium were studied.The 2-phenylethanol was obviously increased by fed-batch for molasses and L-phenylalanine in the Aqueous-polypropylene glycol two-phase culture.Under the optimized two-phase fed-batch conditions,the yield of 2-phenylethanol reached up to 9.03 g/L,increased by 87 % compared with the original 4.82 g/L.The molar conversion rate of L-phenylalanine to 2-phenylethanol rises to 0.82 mol/mol.