研究了树干毕赤酵母菌(Pichia stipitis)对内醚糖的利用及发酵产乙醇的情况。结果表明,与对葡糖糖的利用相比,树干毕赤酵母菌利用内醚糖的效率较低,且不能发酵产乙醇。然而,在内醚糖和葡萄糖同时存在的情况下,内醚糖可促进树干毕赤酵母的生长,乙醇产率也获得提高。在YPD培养基中添加0.1%内醚糖,细胞浓度和乙醇产率最高,其中细胞浓度提高14.3%,乙醇产率提高25.4%。

This investigation focused on the utilization and ethanol fermentation of levoglucosan by the yeast Pichia stipitis.Results revealed that P.stipitis could utilize levoglucosan at a low efficiency compared to glucose,and could not ferment it to ethanol.However,levoglucosan could facilitate the growth of P.stipitis and enhance its ethanol production when both levoglucosan and glucose existed in culture media.In YPD media with 0.1% levoglucosan,the enhancement of cell concentration and ethanol production was approximately 14.3% and 25.4%,respectively.The results would support that levoglucosan could be used as an additive in high cell density fermentation with P.stipitis.