利用水提醇沉法制备刺梧桐粗多糖,经高速离心、酶及sevage联用法除蛋白质及淀粉等杂质,得刺梧桐纯多糖。在BaCl2催化作用下,利用Na2SeO3对刺梧桐多糖进行硒酸化修饰,得刺梧桐多糖硒酸酯,并利用红外、紫外、热重的方法对合成产物进行表征。结果表明:与刺梧桐纯多糖相比,刺梧桐硒酸酯在896.00 cm-1处有一弱吸收峰,归属为C—Se=O的伸缩振动峰,紫外法测定出刺梧桐硒酸酯硒含量为1 747.2 mg/kg。热重分析表明,硒化后,多糖的稳定性降低。

After high speed centrifugation,enzyme treatment combined with sevag method,crude polysaccharide from gum karaya was prepared by water extraction and ethanol precipitation.Under the activation of BaCl2,selenium-polysaccharides were obtained when hydroxy group of polysaccharide of gum karaya was modified by sodium selenite.The final products were characterized by ultraviolet spectroscopy,infrared spectroscopy and thermogravimetric analysis.The results indicated that the products had a weak absorption peak at 896 cm-1 compared to control group,which is attributed to C—Se=O stretching vibration band.The concentration of selenium-polysaccharide was 1 747.2 mg/kg using ultraviolet spectroscopy.Thermogravimetric analysis showed that the stability of selenium-polysaccharidese decreased a little.