建立了短乳杆菌(Lactobacillus brevis)NCL912连续培养生产γ-氨基丁酸的方法。在32℃、pH 5.0、150r/min条件下,通过考察不同稀释率(0.06 h-1、0.08 h-1、0.10 h-1、0.12 h-1和0.14 h-1)时,连续培养体系中菌体浓度、葡萄糖利用和GABA产量3项指标的变化,研究了稀释率对连续培养的影响。结果表明,当稀释率为0.06 h-1和0.14 h-1时,培养不能达到稳态;当稀释率为0.08 h-1、0.01 h-1及0.12 h-1时,反应体系均能进入稳态。在考察的几个稀释率中,0.10 h-1最好。

A continuous culture method of Lactobacillus brevis NCL912 was developed for production of γ-aminobutyric acid.The effects of the dilution rate on the biomass,residual glucose and GABA yielding of the continuous culture were investigated.The results indicated that dilution rate had a significant effect on the cell proliferation and GABA production.Steady state could not be obtained when dilution rates were 0.06 h-1 or 0.14 h-1.When dilution rates were 0.08 h-1,0.10 h-1 or 0.12 h-1,the culture system could reach the steady states.Among the dilution rates tested,0.10 h-1 was the best one for the GABA continuous production.