采用2种商品蔗糖酶水解大蒜渣制备果葡糖浆,经过单因素试验以及正交试验确定了Sigma公司的蔗糖酶水解率较高。其最适酶解条件:pH值为4.8,温度为45℃,底物浓度为8 mg/mL,加酶量为210 U/mL,酶解时间为12 h。在此酶解条件下,蔗糖酶的水解率达到80%,经离子色谱-积分脉冲安培检测法分析,酶解产物以果糖和葡萄糖为主。

Two sucrases were used for high fructose syrup production by hydrolyzing garlic dreg at optimal condition obtained from single-factor and orthogonal experiments.It was found that the high fructose syrup yield of 80% was achieved at pH 4.8,45 ℃,substrate concentration 8mg/mL,and enzyme concentration of 210 U/mL substrate after hydrolyzing for 12h.The hydrolysis products mainly consisted of fructose and glucose determined by ion chromatography-integrated pulsed aperometric detection.