high performance liquid chromatographic(HPLC) method for determination of small molecule polyphenols in hops was established in this paper.The detection limit of this method was under 1.0mg/L,and the recoveries of the polyphenols were all above 92% and relative standard deviation were all bellow 5%,which suggested that the method was accurate.And using the method in determination of 12 polyphenols in 18 kinds of hops.