研究怀牛膝多糖的超声波提取工艺和抗氧化性。在单因素实验基础上采用正交实验得出超声波提取怀牛膝多糖的适宜工艺条件,即超声波功率1 000 W、提取温度60℃、提取时间60 min、料液比(g∶mL)1∶30。在此提取工艺条件下,粗多糖得率为6.1%。体外抗氧化性实验表明,所提取怀牛膝多糖具有较好的抗氧化性,对H2O2、O2-.和.OH的清除率分别可达到75.1%、90.3%和61.3%。

The ultrasonic-assisted extraction technology of crude polysaccharide from Achyranthes bidentata Blume and its antioxidant activities of the polysaccharide were investigated.On the basis of single factor test,extraction technology condition was optimized by orthogonal test.Our results showed the best extracting condition was as follows: ultrasonic power 1 000W,extracting temperature 60℃,extracting time 60 min,and material-water ratio 1∶ 30.Under the above conditions,the extraction rate of the crude polysaccharide can reach to 6.1%.Meanwhile,the scavenging activity of the polysaccharide on H2O2,O-2 · and ·OH free radicals were both significant.The clearance rates was up to 75.1%,90.3% and 61.3% respectively.