研究了脂肪酶促酯交换改性脂质的液-液萃取脱酸工艺,通过单因素实验和响应面法研究了萃取溶液浓度、物料比、萃取次数等工艺条件对改性脂质脱酸率和得率的影响,确定了液-液萃取脱酸的工艺条件。结果表明:改性脂质脱酸率随萃取溶液浓度、物料比、萃取次数的增加而增加,而得率则随这3个工艺条件的增加而减少。由响应曲面法得到的回归模型能很好地反映各因素水平与改性脂质脱酸率和得率之间的关系。两因素对改性脂质脱酸率的影响顺序为萃取溶液浓度>物料比,而两因素对改性脂质得率的影响顺序则相反,物料比>萃取溶液浓度。通过对改性脂质脱酸率和得率的等高线进行叠加可得到液-液萃取脱酸的最佳工艺条件为乙醇水溶液浓度85.00%,物料比为1∶1。

The liquid-liquid extraction process of modified lipid prepared by lipase catalyzed transesterification was investigated.The effects of extraction solution concentration,material ratio,extraction time on free fatty acid extraction ratio and modified lipid yield were investigated by single factor experiment and response surface methodology.The results show that free fatty acid extraction ratio is increasing with extraction solution concentration,material ratio,extraction time increasing,while yield is decreasing with these three process conditions increasing.The regression model from response surface methodology can highly reflect the relationship of different factor levels and free fatty acid extraction ratio,yield.The sequence of two factors influenced on free fatty acid extraction ratio of modified lipid is extraction solution concentration>material ratio,while the sequence of two factors influenced on yield of modified lipid is reversed,material ratio>extraction solution concentration.The optimal process is obtained by overlaying two contour lines of free fatty acid extraction ratio and modified lipid yield,and the optimal process is enthanol aqueous solution concentration 85.00%,material ratio 1∶ 1.