研究了不同植物原料中氢过氧化物裂解酶的含量,并最后筛选出苋菜作为提取原料。利用高速离心、表面活性剂溶解、硫酸铵分级沉淀、羟基磷灰石柱层析和离子交换色谱对苋菜氢过氧化物裂解酶进行纯化至电泳纯。结果表明,纯化的苋菜氢过氧化物裂解酶分子量约为55ku,对于13-亚麻酸氢过氧化物的活力高于13-亚油酸氢过氧化物,最适pH6.0,最适温度为25℃,在常温下酶能保持较高水平的活力。

Amaranthus tricolor leaves were identified as a particularly rich source of HPL activity.Hydroperoxide lyase(HPL) was purified to electrophoretic homogeneity from high speed centrifigation,solubilized by Tritron X-100,ammonium sulfate precipitation,hydroxypatite chromatography and DEAE Toyopearl chromatography.The purified HPL preparation consisted of a single band with a molecular mass of about 55 ku in SDSPAGE.The HPL showed higher activity against 13-hydroperoxy-linolenic acid compared to 13-hydroxy-linoleic acid.Maximum HPL activity was observed at pH 6.0 and 25℃,and room temperature sustains high enzyme activity.