研究了离子色谱法测定糖浆中低聚半乳糖含量的方法。用水、250 nmol/L NaOH溶液和1 mol/L乙酸钠溶液梯度淋洗,选用CarboPac PA10(2 mm×250 mm)色谱柱和安培检测器,测定了糖浆中各组分(乳糖、半乳糖、葡萄糖和低聚半乳糖)的含量。3种糖在测定条件下线性相关系数为0.999 0~0.999 7,相对标准偏差为1.54%~3.42%,样品的加标回收率为91.7%~95.7%。

An ion chromatography method was developed for determination of galacto-oligosaccharide in syrup.The components of syrup was eluted with water,250 mmol/L sodium hydroxide solution and 1mol/L sodium acetate solution in gradient,and using the CarboPac PA10(2 mm×250 mm) column and amperometric detector.The method presented linear correlation coefficient between 0.999 0 and 0.999 7,relative standard deviation(RSD) 1.54 % and 3.42 %.The recoveries of the lactose in syrup sample ranged from 91.7 % to 5.7 %.The method has many advantages,such as simple operation,fast determination,accurate and highly sensitive.