建立超高效液相色谱法(UPLC)测定核桃仁中氨基酸的含量。文中采用Waters Acquity UPLC BEH C18色谱柱(2.1 mm×150 mm,1.7μm),AccQ.Tag Ultraa Eluent A及AccQ.Tag Ultraa Eluent B梯度洗脱,流速为0.7 mL/min,波长248 nm,柱温55℃。结果显示:17种氨基酸在0.003~0.18 mg/mL内有良好的线性关系(r>0.999 4),平均回收率(n=6)为98.5%。该方法10 min内17种氨基酸能够完全分离,具有快速、准确、重复性好的特点。
To establish an UPLC method in determination the amounts of Amino Acids in Walnuts.A Waters Acquity UPLC BEH C18 column(2.1 mm×150 mm,1.7μm)was applied,using AccQ.Tag Ultraa eluent A and AccQ.Tag Ultraa eluent B as the mobile phase,with a flow rate of 0.7 mL/min,and the column temperature 55℃.The calibration curve was linear in the range of 0.003~0.18 mg/mL of Amino Acids(r>0.999 4).The average recovery of Amino Acids was 98.5%.It has the advantage of simple,accuracy,and repeatable in the determination Amino Acids.
