采用生物酶法水解纯化后的麦冬多糖,并对其产物的成分进行分析。利用葡聚糖凝胶Sephadex G-100柱对麦冬粗多糖进行分离纯化,得到相对分子质量为48 347的纯麦冬多糖。使用Absidiasp.O8 s菌产酶水解纯麦冬多糖,产物经Sephadex G-100和Sephadex G-50柱分离,得到OP-1、OP-2、OP-3三种组分,相对分子质量分别为32 452、9 231和1 354。通过高效液相色谱分析纯麦冬多糖及其水解产物的单糖组成,结果表明,纯麦冬多糖的单糖组成为葡萄糖和果糖,其水解产物OP-1、OP-2、OP-3三种组分的单糖组成分别为果糖、果糖、葡糖糖和果糖。

The Ophiopogon japonicus polysaccharide was hydrolyzed by bio-enzyme and the products were analyzed.The relative molecular weight of the Ophiopogon japonicus polysaccharide was 48347,which was purified by Sephadex G-100.The Ophiopogon japonicus polysaccharide was hydrolyzed by enzyme of Absidia sp.O8s,and the products were then isolated using Sephadex G-100 and Sephadex G-50 to generate three components: OP-1,OP-2 and OP-3.Their relative molecular weights were 32452,9231,1354,respectively.From HPLC,the purified Ophiopogon japonicus polysaccharide was mainly composed of glucose and fructose,then,OP-1,OP-2,OP-3 respectively consisted of fructose,fructose,glucose and fructose.