研究了不同溶液提取的板栗壳棕色素的抗氧化性并比较2种栗壳棕色素红外光谱图。用质量分数1%的NaOH溶液和体积分数40%的乙醇溶液分别提取板栗壳棕色素,用总抗氧化性、清除DPPH自由基、清除羟基自由基和清除超氧阴离子4种评价方式,比较碱提栗壳棕色素和醇提栗壳棕色素的抗氧化能力并与通用抗氧化剂VC和BHT做对照。试验结果显示:总抗氧化能力随着样品浓度的增大而增强,VC的总抗氧化能力最强,醇提板栗壳棕色素,碱提栗壳棕色素和BHT的总抗氧化能力比较接近;清除DPPH自由基时,VC清除效果最强,以后依次是醇提栗壳棕色素,碱提栗壳棕色素和BHT,醇提栗壳棕色素的IC50值约为0.02 mg/mL,碱提栗壳棕色素的IC50值约为0.09 mg/mL;清除羟基自由基(.OH)时,随着样品浓度增大对.OH的清除作用增强,碱提栗壳棕色素,醇提栗壳棕色素,BHT和VC的清除效果比较接近;清除超氧阴离子(O2-)时,碱提栗壳棕色素的IC50值约为0.71 mg/mL,醇提棕色素的IC50值约为0.83 mg/mL,清除效果弱于VC和BHT。对2种溶液提取的板栗壳棕色素进行了红外光谱分析,碱提栗壳棕色素和醇提栗壳棕色素的红外光谱图存在差异。

The antioxidant activity of the brown pigment extracted from chestnut shells by two different solutions was compared by IR spectra.The extraction was done by 1% sodium hydroxide and 40% ethyl alcohol respectively.The evaluation was measured by the total antioxidant activity,DPPH radical scavenging ability,hydroxyl radical scavenging capability and superoxide radical scavenging capability.The results show that the samples antioxidant activity increased with the amount of the pigment.Compared with Vc and BHT,Vitamin C showed the highest antioxidant activity.The second is brown pigment extracted by ethyl alcohol.The brown pigment extracted with sodium hydroxide is close to the BHT.The order for DPPH radical scavenging ability is:Vitamin C,brown pigment extracted with ethyl alcohol,brown pigment extracted with sodium hydroxide and BHT.The IC50 of the brown pigment extracted with ethyl alcohol is about 0.02 mg/mL.The IC50 of the brown pigment extracted with sodium hydroxide is about 0.09 mg/mL.For hydroxyl radical scavenging capability,the samples exhibit higher scavenging capability with the increasing of the amount of the samples and the four samples were all very close.The superoxide radical scavenging capability result showed that alkali extraction IC50 was 0.71 mg/mL,the alcohol extraction is about 0.83 mg/mL,both weaker than Vitamin C and BHT.The IR spectra showed the difference between two method extracting pigments.