构建了玉米秸秆两步发酵联产乙醇-氢气体系,系统考察了多种脱毒方法对秸秆稀酸水解物发酵生产乙醇和氢气的影响,探讨了秸秆的降解机理。结果表明:当以稀酸水解玉米秸秆时,采用离子交换树脂吸附结合真空旋转蒸发对玉米秸秆稀酸水解液进行脱毒处理效果最好,乙醇和氢气的最大产量分别为131.1 mg/g TS和44.6 mL/g TS,其秸秆的能量回收率为33.2%。玉米秸秆经稀酸处理后绝大多数半纤维素被降解用于发酵生产乙醇,而天然产氢微生物具有直接降解纤维素发酵产氢的能力。

A two-phase process combined production of cellulosic ethanol and hydrogen from cornstalk was constructed in this paper.The effect of detoxification methods on the production of ethanol and hydrogen from acid-hydrolysis cornstalk were investigated.The results showed that using anion-exchange resins detoxification followed by evaporation,the maximal ethanol and hydrogen yields from cornstalk were 131.1 mg/g TS and 44.6 mL/g TS,respectively.Using acid hydrolysis method,the hemicellulose in cornstalk was degraded for ethanol production.The natural hydrogen-producing microorganism had the ability to directly degrade cellulose for hydrogen fermentation.