将酵母破碎液在红曲霉接种培养后的不同时间点及在最适时间点以不同体积加入,探讨酵母破碎液加入时间及加入量对红曲色素生产的影响。结果表明,500 mL三角瓶(装液量为150 mL)在接种后的第48 h加入1.5 mL酵母破碎液,可以促进菌体生产,同时提高色素产量。菌体量、醇溶性色素、水溶性色素增幅分别达到47%、120%和190%。

The production of red pigment was greatly improved by Monascus incubated with Saccharomyces cerevisiae lysate.Yeast lysate was added at different time points to the different flasks which contained media inoculated with Monascus and the difference of the red pigment yield was investigated.The effect of the amount of the yeast lysate added on the red pigment yield was also analyzed.The results shows that 48 hours after inoculating with Monascus is the most suitable time point to add yeast lysate and 1.5 mL is the most suitable amount of yeast lysae for 150 mL culture mix to increase the pigment yield.Under the most suitable condition mentioned above,the rates of increase in yield of Monacus mycelium,hydrophobic pigment and hydrophilic pigment were 47%,120% and 190%,respectively,as compared with control.