以玉米皮水不溶性膳食纤维为原料,对复合纤维素酶法制备水溶性膳食纤维(SDF)进行了研究。采用六偏磷酸钠及高温蒸煮等处理方法强化酶解过程以提高水溶性膳食纤维得率。结果表明,高温蒸煮有助于提高玉米皮水溶性膳食纤维得率,条件为121℃,3 h。在单因素实验的基础上,采用L9(34)正交实验对复合纤维素酶法制备水溶性膳食纤维的条件进行优化。结果表明,玉米水溶性膳食纤维的较佳提取条件为:复合纤维素酶的添加量2%,底物浓度40 g/L,酶解温度55℃,pH4.0,酶解11 h。在此条件下,玉米SDF得率达到10.37%。

Soluble dietary fiber was obtained from insoluble dietary fiber of corn bran by treatment with a celluclast.Insoluble dietary fiber is subjected to a sodium hexametaphosphate solution or high pressure treatment in order to enhance enzymic access and soluble dietary fiber production.The results showed that high pressure treatment was most useful to increase the yield of soluble dietary fiber,and high pressure treatment condition was 121℃,3 h.The reaction parameters affecting the soluble dietary fiber,such as substrate concentration,enzyme loading,pH,reaction temperature and reaction time,have been investigated.The maximum soluble dietary fiber production was observed after 11 h incubation of enzyme loading 2%,substrate concentration 40 g/L,temperature 55 ℃,and pH 4.0.The obtained yield of soluble dietary fiber was 10.37%.