采用不同方法从苹果中提取正己醇降解酶,优选最适的提酶方式,并在该提取方式下研究酶的初步分离纯化。结果显示:采用丙酮粉法提酶较适合,且SDS-PAGE电泳结果表明不同方法提取粗酶液和经硫酸盐处理后的蛋白在条带数量和分布上都有所变化。30%~70%为适宜的(NH4)2SO4盐析浓度,然后对酶液进行梯度盐析,初步纯化后正己醇降解率为88.56%±0.9847%。

In this paper,different extracting methods of Hexanol degradating enzyme from apple were investigated for purpose of comparison,and the best extracting method was obtained.Then crude enzyme extracted with above suitable method was separated and purified for further purification.Results showed that: enzyme extraction with acetone was the best;the SDS-PAGE indicated the electrophoretic bands and molecular weight(MW) of the enzyme obtained through different measures were different from each other.The suitable concentration for ammonium sulphate fractional salting out is 30%~70%,and the Hexanol conversion rate is up to(88.56±0.984 7) % by means of gradient salting out.