对酿酒酵母CWY132发酵罐小试生产2-苯乙醇发酵工艺中的pH、溶氧水平和补糖方式等参数进行了研究。实验表明,最适培养条件为初始pH5.5,培养过程中不需要调节和控制pH值;在细胞生长阶段维持较高的溶氧,在稳定期降低溶氧量对2-苯乙醇的合成具有显著促进;通过补糖方式逐步添加葡萄糖有利于提高2-苯乙醇的产量。在优化的培养工艺条件下,2-苯乙醇产量达到最高为4.1 g/L,已是目前报道的酿酒酵母能够耐受的最大浓度,摩尔转化率为79.43%。

Through a small desktop automatic fermentor bio-transformation test,the pH,dissolved oxygen and other parameters which affect the production of 2-phenylethanol(2-PE) were studied.The results showed that the initial pH5.5 of medium was suited and not need to be further controlled,and dissolved oxygen was taken different actions during different phase;batches up the sugar could promote the growth of S.cerevisiae CWY132 and greatly increase the production of 2-PE.Under the optimum culture conditions,the production of 2-PE reached 4.1 g/L,the molar conversion rate of L-phenylalanine to 2-PE rises to 79.43%.