在优化了批式发酵条件的基础上,通过对流加补料方式、补料速度等发酵过程的各种参数,包括产酸率、转化率、发酵周期等进行了研究,优化了L-鸟氨酸补料分批发酵的条件。在最优补料分批发酵条件下,发酵58 h,L-鸟氨酸产量达59.35 g/L,糖酸转化率达37.09%。发酵结果明显优于分批培养。

Investigation for effect of feed model,start feed time,feed speed on parameters of fermentation process such as concentration of L-ornithine,conversion rate of glucose,fermentation period in the fermentation broth were carried out by optimization of batch fermentation.The optimum fermentation condition was determined.In the optimum batch fermentation condition,the yield of L-ornithine was 59.35 g/L and the conversion rate was 37.09%.