将燕麦种子进行发芽,对未发芽原样、发芽6 d的籽粒、芽和根分别用体积分数80%的甲醇、乙醇和丙酮溶液提取其多酚,测定了不同溶剂提取液的总酚提取率、清除DPPH.自由基的能力、还原能力以及清除亚硝酸盐的能力,并对其差异性及相关性进行了分析。结果表明:发芽前后燕麦多酚均具有较强的清除DPPH.自由基的能力及较强的还原能力,对亚硝酸盐也具有一定的清除作用。不同溶剂提取液的抗氧化活性与其总酚种类和极性密切相关,发芽在一定程度上提高了燕麦的抗氧化活性。
Oat seeds were steeped and germinated.Polyphenol compounds were extracted by 80% methanol,ethanol and acetone solvent respectively from non-germination oat,germinated seed,bud and root after 6 d's germination.Total phenolic yield,reducing power,scavenging activity to DPPHo radical and nitrite have compared.The results indicated that oat polyphenols had strong reducing power and scavenging effects to DPPHo radical and nitrite in both germinated and non-germinated seeds.The antioxidant activity of different solvents extracts was closely related to the kind and polarity of oat polyphenols.The antioxidant activity was higher in the germinated seeds.