建立了间接竞争化学发光酶免疫法检测赭曲霉毒素A(ochratoxin A,OTA),该方法IC50为112 pg/mL,检出限是2.47 pg/mL,平均批内和批间变异系数分别为7.03%和14.7%。在大米和小麦样本中添加浓度1.5～6μg/kg的OTA标品,平均回收率在66.97%～97.96%之间,与其他常见真菌毒素未见交叉反应。将该方法应用于30份谷物样本(包括20份大米样本和10份小麦样本)中OTA的检测,检测结果与商品化ELISA试剂盒的相关系数R2=0.942 4。该方法简单、灵敏、快速、准确适用于谷物中OTA的检测。

A sensitive indirect competitive chemiluminescence enzyme immunoassay(CLEIA)for OTA was developed.The concentration of OTA causing 50% inhibition of binding enzyme marker(IC50) was 112 pg/mL and the detection limit was 2.47 pg/mL,the mean coefficients of variation of within groups and between groups were 7.03,14.7% respectively.The recoveries from rice and wheat samples spiked with 1.5~6 μg/kg of OTA varied between 66.97% and 97.96%,and the OTA antibody showed no cross-reactivity to the else common mycotoxins.OTA in 30 cereal samples were screened by the CLEIA,including 20 rice samples and 10 wheat samples.The results show good coefficient with commercial ELISA kit(R2= 0.9424).The assay was sensitive,fast and accurate,which proved to be suitable for the screening of cereal samples for the presence of OTA.