与平菇单菌发酵相比,玉米秸秆经平菇与产朊假丝酵母混菌固态发酵后,其基质粗蛋白含量由8.13%提高到13.41%。为进一步提高混菌固态发酵玉米秸秆基质的粗蛋白含量及木质素酶系的活性,在基础培养基中分别添加豆渣(2%、3%、4%)、(NH4)2SO4(1%、2%、3%)、CuSO4.5H2O(0.04、0.4、4 mmol/L)、MnSO4(0.001、0.01、0.1 mmol/L)。结果表明:所试浓度的豆渣和(NH4)2SO4、0.01 mmol/L MnSO4均显著提高基质的粗蛋白含量,其中2%的豆渣、3%的(NH4)2SO4效果较好;所试浓度的豆渣、(NH4)2SO4和MnSO4均显著提高漆酶、锰过氧化物酶的活性,0.04、0.4、4 mmol/L CuSO4.5H2O显著提高漆酶的活性,而只有0.4和4 mmol/L的CuSO4.5H2O显著提高锰过氧化物酶的活性,其中2%的豆渣、3%的(NH4)2SO4、0.4 mmol/L的CuSO4.5H2O和0.01 mmol/L的MnSO4对漆酶活性提高的幅度最大,当添加的豆渣、(NH4)2SO4、CuSO4.5H2O和MnSO4的浓度分别为3%、3%、4 mmol/L和0.01 mmol/L时,对应的锰过氧化物酶活性最高。漆酶的活性与发酵基质的粗蛋白含量可能呈正相关关系。

Compared to the single-strain fermented of Pleurotus ostreatus,the crude protein content of the corn straw substrate was increased from 8.13% to 13.41% when it was fermented by mixed-strain of P.ostreatus and Candida utilis.Different concentrations bean dregs powder(2%,3%,4%),(NH4)2SO4(1%,2%,3%),CuSO4·5H2O(0.04,0.4,4 mmol/L) and MnSO4(0.001,0.01and 0.1 mmol/L) were added to the basic medium for the further increasing of the crude protein content and the activity of ligninolytic enzyme system.The results showed that: bean dregs powder and(NH4)2SO4 at the all experimental concentrations,and 0.01 mmol/L MnSO4 increased significantly the crude protein content of the corn straw substrate,and the increasing effects were best when the concentration of bean dregs powder and(NH4)2SO4 were 2% and 3% respectively;bean dregs powder,(NH4)2SO4 and MnSO4 at the all experimental concentrations increased significantly the activities of lactase(Lac) and manganese peroxidase(MnP),0.04,0.4,4 mmol/L CuSO4·5H2O and 0.4,4 mmol/L CuSO4·5H2O increased significantly the activities of Lac and MnP respectively.The activity of Lac was at its peak when the concentration of bean dregs powder,(NH4)2SO4,CuSO4·5H2O,MnSO4 were 2%,3%,0.4 mmol/L and 0.01 mmol/L respectively,and the activity of MnP was the highest when the concentration of bean dregs powder,(NH4)2SO4,CuSO4·5H2O,MnSO4 were 3%,3%,4 mmol/L and 0.01mmol/L respectively.The correlation between the activity of Lac and the crude protein content of the fermented corn straw substrate was probably positive.