为研究松花粉抗氧化活性,采用HPLC-ECD方法测定了松花粉的水提取物和乙醇(乙醇体积分数为80%)提取物中的酚类化合物的含量。同时评价了其对羟基自由基介导的2-脱氧核糖裂解的保护作用。结果表明:2种提取物中均含有原儿茶酸、咖啡酸、p-香豆酸、阿魏酸和高良姜素,乙醇提取物中含有芦丁、槲皮素和山奈酚。松花粉乙醇提取物中的总酚含量显著高于水提物(P<0.05),但水提取物的还原能力和络合能力显著高于乙醇提取物(P<0.05)。2种提取物对非定位羟基自由基介导的2-脱氧核糖裂解的保护作用均显著高于定位羟基自由基(P<0.05),表明松花粉提取物对羟基自由基介导的2-脱氧核糖裂解的保护作用主要通过清除羟基自由基来实现的,这可能与松花粉提取物中含有大量的酚类化合物有关。

Antioxidant activities of the pine pollen extracts was studied.Phenolics were extracted from the pine pollen using water and 80% ethanol as solvents.The extracts are analyzed by HPLC-ECD.And the scavenging effects of extracts on hydroxyl radical-mediated 2-deoxyribose degradation were studied.The results revealed that protocatechuic acid,caffeic acid,p-coumaric acid,ferulic acid and galangin could be identified and quantified in both extracts.Rutin,quercetin and kaempferol were identified and quantified in ethanol extracts.The total phenolic content of ethanol extracts was significantly higher than water extracts(P<0.05).Reducing power and chelating activity of water extracts were significantly higher than ethanol extracts(P<0.05).The both extracts performed better in the nonsite-specific assay than in the site-assay(P<0.05).It was also found that pine pollen extracts had higher scavenging activity than iron chelation ability for hydroxyl radicals.This may due to the high level of phenolic compounds in the pine pollen.