将鳕鱼皮打浆后利用米曲霉进行液态发酵以制取有较高价值的低分子量多肽,从碳氮比、装液量、底物浓度、转速、碳源、表面活性剂、不同起始pH、接种量8个方面进行了单因子实验。进而采用响应面法(Response Surface Methodology,简称RSM)进一步优化影响发酵的最重要因素:碳氮比、底物浓度、接种量,得到发酵的最优条件,即发酵中碳氮比应控制在0.29,底物浓度为7.0%,接种量为4×107孢子/100 mL,此时发酵水解度可以达到36%,对发酵肽的分子量进行测定,发现分子量1 000 u以下小肽占到71.5%。同时测定了多肽产物的抗氧化能力和降血压活性,其DPPH抑制的IC50值为1.3 mg/mL,其血管紧张素转化酶抑制的IC50值为2.53 mg/mL,因此证明所得发酵肽不仅分子量低,且具有良好的抗氧化能力和降血压活性。

The peptides was obtained by submerged fermentation of cod fish skin after which was cracked with Aspergillus oryzae utilized,The optimization was started from 8 aspects-the carbon and nitrogen ratio,liquid volume,substrate concentration,rotation speed,carbon resource,surfactants,different initial pH,inoculation rate,after which the most important factors was chosen-carbon and nitrogen ratio,inoculum,substrate concentration to do the response surface experiment.The optimum fermentation conditions were as follows: 0.29 ratio of carbon and nitrogen,7.0%substrate concentration,inoculum size 4×107,then the ratio of hydrolysis could come up to 36%,the molecular weight below 1000 took up about 71.5% of fementation peptide.This paper also monitored the activity of antioxidation as well as antihypertense of the peptides,which proved that the IC50 of DPPH could be 1.3 mg/mL,The IC50 of anti-ACE of peptides was 0.88 mg/mL,the peptide of this paper was in low molecular weight with high activity of antioxidation and antihypertense.