采用超声提取法提取荷叶中的O-去甲荷叶碱、N-去甲荷叶碱、荷叶碱和莲碱4种主要的生物碱,建立HPLC分析方法进行评价,为荷叶及相关产品的质量控制提供参考。选用Welch Materials C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(含0.1%三乙胺)为流动相进行梯度洗脱,实现了O-去甲荷叶碱、N-去甲荷叶碱、荷叶碱和莲碱4种生物碱的同时基线分离。在0.1～50μg/mL范围内,O-去甲荷叶碱(R=0.999 8)、N-去甲荷叶碱(R=0.999 9)、荷叶碱(R=0.999 8)和莲碱(R=0.999 8)具有良好的线性关系,检出限分别为1.87、2.22、2.34和1.88 ng/mL(S/N=3∶1)。

Using ultrasonic extraction,O-nornuciferin,N-nornuciferin,nuciferine and roemerine in Nelumbo nucifera leaves were extracted and determined by the established HPLC method.This work provided a new method for quality control of Nelumbo nucifera leaves and its products.Four alkaloids were separated on a reversed-phase Welch Materials C18 column with a gradient elution using acetonitrile-1% triethylamine solution as mobile phase.Linear regression of O-nornuciferin,N-nornuciferin,nuciferine and roemerin in the concentration range of 0.1~50 μg/mL(R>0.999) was satisfied and the detection limit were 1.87 ng,2.22ng,2.34ng and1.88 ng(S/N=3∶ 1),respectively.The method was very precious with good recovery.It can be used for determination of alkaloids from Nelumbo nucifera leaves.