用人体粪便作为肠道细菌来源,分离不同肠道菌群利用低聚异麦芽糖作为碳源进行体外发酵培养,在不同时间取其发酵液,利用气相色谱法测定发酵液内短链脂肪酸(SCFA)的种类和数量的变化。结果显示,肠道内主要是双歧杆菌、拟杆菌、肠杆菌、乳酸菌和肠球菌菌群,它们都能利用低聚异麦芽糖发酵产生SCFA,但产量和种类各不相同。发酵12 h后,低聚异麦芽糖(IMO)对乙酸的贡献值最大,而丁酸和异丁酸的产量都很少甚至检测不到。

Oligosaccharide can be fermented at human colon by gut microbes to produce short chain fatty acids.This paper took isomalto-oligosaccharide(IMO) as carbon source to in vitro ferment with different gut microbiota that come from human feces.samples were collected at different time during fermentation to determine the volume and the types of short chain fatty acids.The results show that all of Bifidobacterium,Bacteroides,Enterobacteria,Lactobacillus and Enterococcus can utilize IMO to produce short chain fatty acids that are healthy for human body,but their volume and types are different.After 12 h fermentation,the volume of acetic acid is the most than other acids and however butyric acid and isobutyric acid are too little to determine.