采用有机微滤膜对结冷胶发酵液进行过滤除菌实验。考察了不同膜材料的过滤性能,以及结冷胶浓度、温度、压力等操作参数对除菌效果的影响。将结冷胶发酵液脱酰基处理后稀释成浓度为3 g/L的料液,再将酸碱度调至pH10,在0.1 MPa、65℃下用孔径为0.45μm的聚醚砜膜过滤,菌体去除率和结冷胶回收率分别为97%和84%。滤膜污染后采用0.75%HCl和1%NaOH交替清洗,可使膜通量得到较好地恢复。

Organic membranes were used to remove cells from deacetylated gellan gum by microfiltration.The performance of different organic membranes and the operating conditions such as feed concentration,temperature,pressure were studied.The optimum conditions were as follows: 3 g/L deacetylated gellan gum,pH 10,65℃,0.1MPa.The results showed that the cell removal and gellan gum recovery were 97% and 84%,respectively by microfiltration using PES membrane with a pore size of 0.45μm.The flux of foul membrane can be well recovered by washing the membrane with 0.75% HCl and 1% NaOH alternately.