以研究羊胰脏提取胰酶工艺参数为目的。用低浓度异丙醇做提取剂,高浓度异丙醇做沉淀剂,在单因素基础上,运用均匀设计法研究激活时间、沉淀剂浓度、提取pH值和CaCl2对羊胰酶提取工艺的影响。胰蛋白酶、胰淀粉酶、胰脂肪酶活力和胰酶提取率为响应指标,通过偏最小二乘回归(PLS)分析法对胰酶制备工艺的影响因素进行优化组合,并建立了具有制备条件的数学模型。试验结果表明,羊胰酶制备最适条件为:激活时间12 h、沉淀剂浓度41%、提取pH值6.34和CaCl2 0.08%。此条件下验证实测值胰蛋白酶、胰淀粉酶、胰脂肪酶的活力和胰酶提取率分别为3 062.78 u/g、30.06 ku/g、32.37 ku/g和6.16%,与理论预测值的相对误差分别为8.11%、1.28%、3.55%和5.29%。进一步验证了数学二次多项式回归模型的适合性,PLS优化结果的合理性。

The purpose of this paper is to optimize the conditions for extraction of pancreatic enzyme from the ovine pancreas.With low concentration of isopropanol for extraction and high concentration of isopropanol for precipitant,the effects of activating time,precipitant concentration,extracting pH and amount of CaCl2 on pancreatin preparation were studied by the means of uniform design on the basis of a single factor experiment.With the extracting rates of trypsin,amylase and lipase as responding results,the factors for pancreatin preparation were optimized by partial least square(PLS) regression analysis.The results showed that the optimal processing conditions for preparation of sheep pancreatic enzymes: activating time 12h,precipitant concentration 41%,extracting pH 6.34 and CaCl2 0.08%.Under the conditions,activities of trypsin,amylase and lipase were 3 062.781u/g,30.06 ku/g,32.37 ku/g respectively and the extract rate was 6.16%.The relative errors of the model values were 8.11%,1.28%,3.55%,5.30%,respectively.These showed the suitability of the mathematical quadratic polynomial regression model and the rationality of PLS optimization results.