对高效液相色谱法测定蜂胶类食品中吡啶甲酸铬含量的前处理方法进行了改进。准确称取一定量试样于容量瓶中,加入纯甲醇定容至100 mL,室温下超声提取30 min,然后用高效液相色谱-二极管阵列检测器测定其中的吡啶甲酸铬含量。采用改进方法吡啶甲酸铬的线性范围2～200μg/mL,加样回收率为87.3%(RSD<1.6%)。

An improved method for the detection of chromium picolinate in propolis healthy food by HPLC was established.Sample was added into calibrated flask and dissolved with 100ml methnol.After ultrasonic treatment for 30min,the content of the chromium picolinate in the food was detected by HPLC with diode array detector.The results showed a good literary(r>0.999 5) of the improved method in the test range of 2~200 μg/mL and high recovery of 87.3 %(RSD<1.6%).It is concluded that the content of chromium picolinate in the propolis healthy food could be accurately detected and this provides a method for the quality control.