利用菊粉酶酶解法提取菊芋渣中的单糖,采用单因素实验和正交实验研究提取工艺条件,优化的提取条件为:pH值4.6、酶加量25 U/g、提取温度60℃、料液比1∶25、提取时间6 h。在此条件下,单糖平均提取率为82.67%。采用薄层色谱法对酶解产物进行成分分析,结果表明,酶解液中主要含有果糖和葡萄糖两种单糖。经DNS法测定,酶解1 g底物,产物中果糖和葡萄糖的含量分别为135.9 mg和84.9 mg,两种单糖的比例为1.6∶1。

To optimize the extraction of monosaccharides from Jerusalem artichoke residue based on inulinase treatment,the single factor test and the orthogonal test were used.The results showed that the optimum conditions are pH4.6,enzyme dosage 25U/g,extraction temperature 60℃,liquid ratio 1∶ 25,the extraction time 6h.Under these conditions,the average extraction yield of monosaccharide is 82.67%.The hydrolysate contains mainly two kinds of simple sugars,fructose and glucose by TLC analysis.And the contents of two monosaccharides were 135.9mg/g and 84.9mg/g using the DNS method,the ratio of the two sugars was 1.6∶ 1.