以黒粒小麦麸皮为原料,应用高效液相色谱配以串联质谱和二极管阵列检测技术对黒粒小麦中麸皮中的花色苷的组成进行了分析。结果显示:从黒粒小麦麸皮中分离鉴定出9种不同的花色苷类化合物———矢车菊素-己糖苷、矢车菊素-芦丁苷、芍药素-己糖苷、矢车菊素-丙二酰葡萄糖苷、飞燕草素-己糖苷、飞燕草色素-芦丁苷、锦葵色素-芦丁苷、芍药素-芦丁苷及牵牛花素-芦丁苷,其中飞燕草类花色苷和矢车菊素类花色苷是主要花色苷,分别占全部花色苷含量的50.27%和30.04%。

Tandem mass spectrometry(MS/MS) coupled with high performance liquid chromatography(HPLC) with photo-diode array detection(PDA) method were used to analyze and identify the anthocyanins from black wheat bran.Results indicated that nine anthocyanins were identified in black wheat bran:cyanidin-glucoside,cyanidin-rutinoside,peonidin-glucoside,cyanidin-malonyl glucoside,delphinidin-glucoside,delphinindin-rutinoside,malvidin-rutinoside,peonidin-rutinoside and petunidin-rutinoside.Delphinium and peonidin anthocyanins are the major anthocyanins,the percentage of their content was 50.27% and 30.04% respectively in the total pigment.