以海地瓜为实验原料,选用大孔树脂对海参多肽进行脱色。通过比较6种大孔树脂对海地瓜多肽的脱色效果,从中筛选出效果最好的大孔阴离子交换树脂D392;利用响应面实验构建D392脱色的数学模型,确定其脱色工艺为:pH值8.5、温度44℃、树脂用量6.5 g/g(处理液固形物)、处理液浓度4%、处理时间3 h,此时脱色率达(83.2±1.0)%,多肽保留率为(80.5±1.9)%。

In this study,Acaudina molpadioides was used as experimental materials,and for the first time,macroporous resins was used in decolorization of peptides.The decolorization effects of peptides from Acaudina molpadioides using 6 kinds of macroporous resins were compared.Anion exchange resin D392 was the best among them.A mathematic model for the decolorization progress of D392 via response surface experiments was established.The best decolorization technology was as follows: pH 8.5,temperature 44℃,6.5g D392 per gram of the solid,the peptides concentration 4% and treating for 3 hours.The decolorization rate using resin D392 was(83.2±1.0)%,and the peptides reserved rate was(80.5±1.9)%.This article provides an ideal proposal for the decolorization of sea cucumber peptides,improve the quality of the peptides and the references for its further use and study.