研究了阿卡波糖产生菌游动放线菌A-56在30 m3罐上的发酵放大策略。首先通过摇瓶分批补料发酵,考察了补料培养基中麦芽糖和葡萄糖配比对阿卡波糖合成的影响,结果表明,麦芽糖和葡萄糖的配比对阿卡波糖的生物合成具有显著的影响,且麦芽糖和葡萄糖的配比为3∶1最利于阿卡波糖合成;在此基础上,在100 L发酵罐上进一步考察了碳源控制对阿卡波糖发酵的影响,结果表明,补料阶段的发酵液总糖和还原糖浓度分别控制在75～80 g/L和45～50 g/L时,最利于阿卡波糖合成。因此,筛选出总糖和还原糖(麦芽糖和葡萄糖)这两个关键参数作为放大因子,成功地实现了阿卡波糖从100 L罐到30 m3罐的工业化发酵放大,最终(168 h)的阿卡波糖产量达到4 327 mg/L。

The scale-up strategy for acarbose fermentation by Actinoplanes sp.A-56 was explored in this paper.The results obtained in shake-flask cultivation demonstrated that the ratio of maltose and glucose had significant effect on acarbose biosynthesis,and the feeding medium containing 3∶ 1(mass ratio) of maltose and glucose was favorable for acarbose production.Then the correlation of carbon source concentration with acarbose production was further investigated in 100 L fermenter,and the results showed that 75~80 g/L of total sugar and 40~45 g/L of reducing sugar were the optimal for acarbose production.Based on the results in 100 L fermenter,an effective and simplified scale-up strategy was successfully established for acarbose fermentation in a 30 m3 fermenter by using total sugar and reducing sugar as the scale-up parameter.As a result,4 327 mg/L of acarbose was obtained at 168h of fermentation.