利用果胶酶、纤维素酶和漆酶对香菇进行酶解,固定料水比(1∶15)和反应温度(40℃),以酶解后多糖提取率为指标,考察不同浓度的3种酶对香菇多糖提取的影响。在单因素试验的基础上,采用响应面法建立香菇多糖酶法提取的二次多项数学模型,并验证了该模型的有效性;探讨了果胶酶、纤维素酶、漆酶3个因子的交互作用及其最佳水平。响应面分析结果表明:果胶酶、纤维素酶、漆酶显著影响香菇多糖的提取效果,在果胶酶浓度为7.11%,纤维素酶浓度为6.82%,漆酶浓度为3.69%的优化条件下,香菇多糖的提取率达到37.07%。

Effects on polysaccharide yield of enzyme digestion with different concentrations of pectinase,cellulase,laccase at fixed substrate concentration(1∶ 15) and reaction temperature(40℃) were investigated.Based on results from university test,response surface method was applied to establish the quadratic polynomial model and the validity was verified.Interactions among these three factors of pectinase,cellulase,laccase and their optimal level were discussed.Results of response surface analysis showed that: pectinase,cellulase,laccase significantly affects lentinan yield independently and a 37.07% extraction ratio of lentinan was achieved with the optimized concentration of three enzyme at 7.11%,6.82% and 3.69%,respectively.