初步分析了水酶法工艺得到的菜籽水解蛋白的组成、体外抗氧化活性和抑制ACE活性,并用凝胶过滤色谱法分离水解蛋白得到不同组分,探讨了不同组分的体外抗氧化活性和抑制ACE活性与组分氨基酸组成、分子量分布的关系。结果表明,水解蛋白具有良好的体外功能性质,经Sephadex G-25分离后的9个峰中,峰2、峰4和峰5具有较好的体外功能性质,它们的分子量分布中90%以上低于1000,且含有较高量的疏水性氨基酸。

The composition,antioxidant activity in vitro and inhibition of ACE activity of rapeseed protein hydrolysates(RPH) were analyzed in this paper.The protein hydrolysates were separated by gel filtration chromatography into different components.The relationship of antioxidant activity in vitro and inhibition of ACE activity of different components between the amino acid composition and the molecular weight distribution of the components were discussed.The result shows that the protein hydrolysates have good functional properties in vitro.Among nine peaks separated by Sephadex G-25,peak 2,4 and peak 5 have better functional properties in vitro;these peaks contain higher levels of hydrophobic amino acids and more than 90% of the molecular weight distribution which is lower than 1000.