通过实验比较XDA-7、AB-8、D101、DA-201、X-5五种树脂对玫瑰茄红色素的纯化效果,实验表明XDA-7树脂对玫瑰茄红色素的吸附选择性最佳、纯化效果最好。通过单因素试验和正交试验,优化XDA-7树脂纯化玫瑰茄红色素的工艺条件,并且通过红外对玫瑰茄红色素的结构进行初步鉴定。结果表明:XDA-7树脂纯化玫瑰茄红色素的适宜吸附工艺条件为,上样浓度2 mg/mL、上样流速1.75 mL/min、pH值2.76;解吸工艺条件为,洗脱剂用量60 mL、乙醇体积分数75%、洗脱剂pH值3.5、洗脱流速1.5 mL/min。纯化后,玫瑰茄红色素的色价为52.6,是未纯化的玫瑰茄红色素的7倍左右,初步推断玫瑰茄红色素为矢车菊-葡萄糖苷类或者飞燕草-葡萄糖苷类色素。

After comparing the adsorption and desorption rate of XDA-7,AB-8,D101,DA-201,X-5,XDA-7 was selected as the optimum resin to purify the roselle red pigment.The purifying processing conditions of roselle red pigment was confirmed by the single factor and orthogonal test,and the structure of the roselle red pigment was identified by IR.The optimum absorption conditions were :sample 2mg/mL,the flow rate 1.75mL/min,pH 2.76.The optimum desorption conditions were : the volume of elution 60mL,concentration of eluent 75%,pH of eluent 3.5,elution rate 1.5mL/min.After the purification,the color value of roselle red pigment was 52.6,which was as much as 7 times of unpurified pigment.The structure of the roselle red pigment was identified by IR.The main structure of the pigment was cornflower-glucosides or delphinium-glucosides by IR.