以出芽短梗霉色素低产株G58-2为生产菌株,研究了其透析培养下的菌体形态,及普鲁兰多糖摇瓶发酵的最佳条件。结果表明,通过透析培养制备的发酵接种液优于普通培养,在其OD600为0.442,接种龄36h,初始pH7.0,装液量50 mL/250 mL,摇床转速200 r/min条件下培养84 h出芽短梗霉G58-2不仅色素含量低,粗多糖产量可达到4.84 g/100 mL,并且实验稳定性有效提高。

In this paper,the cell morphologies of Aureobasidium pullulans G58-2 under the dialysis cultivation,and the optimal cultivation conditions for pullulan production were studied.The results showed that inoculation by dialysis cultivation was better than normal cultivation,OD600 of bacterial suspension was 0.442,the inoculation time 36 h,initial pH7.0,250 mL shake flask containing 50 mL of sucrose medium with 200 r/min.Under these conditions,the highest pullulan yield(4.84 g/100 mL) with the lowest pigment was obtained,and the stability of experiments effectively was improved.